DEAE Sephacel
DEAE Sephacel
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DEAE-Sephacel, diethylethylaminoethyl, Sephacel DEAE-Sephadex, diethylethylaminoethyl, Sephadex PEI-cellulose, polyethyleneimine, cellulose weak cation
A protein binds to phosphocellulose column at pH and elutes at pH If the protein has to be further purified on a DEAE Sephacel column, the binding chromatography on DEAE-Sephacel column An immedi- ately eluted nonbound protein fraction and the fraction eluted with M NaCl in 20 mM
pg near hindustan university chennai Ion-exchange chromatography of 17* on DEAE Sephacel Inset: FPLC analysis of the end peak from DEAE DEAE-cellulose is now itself also available in a bead form called DEAE-Sephacel The Sephadex and Sepharose types are particularly useful for the separation of