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Detailed instructions on reagent preparation and assay protocol for an MTT assay to measure cell proliferation or cell cytoxicity  Final concentration of MTT is mgml • Use 1xPBS to dissolve the MTT powder • Filter it before use Procedure 1 After incubation of cells

west bengal lottery result 1pm In the 96-well microplate, 100–200 μl of diluted semen sample and MTT stock solution were placed in each well, at an approximate Following reconstitution, aliquot and freeze Stock solutions are stable for up to 1 month at -20°C Warning Toxicity: Harmful & Carcinogenic